Loss of 5-hydroxymethylcytosine and TET2 in oral squamous cell carcinoma.

نویسندگان

  • Fredrik Jäwert
  • Bengt Hasséus
  • Göran Kjeller
  • Bengt Magnusson
  • Lars Sand
  • Lena Larsson
چکیده

AIM Epigenetic modifications, such as DNA methylation, are considered important in the regulation of target genes in cancer development. 5-Hydroxymethylcytosine (5hmC) was recently discovered to be related to the process of malignant transformation. The influence of DNA methylation in oral squamous cell carcinomas (OSCC) is not fully-understood. Therefore, the aim of the present study was to investigate the DNA methylation pattern in OSCC compared to healthy oral epithelium. MATERIALS AND METHODS Oral mucosal samples from patients with OSCC (n=15) and healthy mucosa (n=12) were analyzed using immunohistochemistry with antibodies against 5hmC, 5mC and ten-eleven-translocation-2 (TET2). RESULTS A significant decrease in 5hmC and TET2 expression was found in OSCC compared to healthy oral epithelium. In contrast, there was a significant increase in 5mC expression in OSCC compared to healthy epithelium. CONCLUSION Our results indicate that loss of 5hmC is an epigenetic event of OSCC.

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عنوان ژورنال:
  • Anticancer research

دوره 33 10  شماره 

صفحات  -

تاریخ انتشار 2013